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    Structured Review

    DSMZ paper zenodo data
    Paper Zenodo Data, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+zenodo+data/HCC-33/pmc12116416__mmc14-898-203-217
    Average 93 stars, based on 7 article reviews
    paper zenodo data - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Staining:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Gel Extraction:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Polymerase Chain Reaction:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Purification:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Ligation:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Luminescence Assay:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Sequencing:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Generated:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    RNA Sequencing:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Chromatin Immunoprecipitation:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025

    Mouse Assay:

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025 Article ll OPEN ACCESS

    Article Title: Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel VWR Cat# 734-0270 Doxycyline-supplemented diet SSniff Cat# A115D70541 Accutase Sigma Aldrich Cat# A6964 Insulin Merck Cat# I9278 Transferrin Merck Cat# T8158 hydrocortisone Merck Cat# H0888 sodium selenite Merck Cat# S5261 b-estradiol Merck Cat# E2758 Primocin Invivogen Cat# ant-pm-2 ROCK inhibitor Tocris Cat# 1254-10 DNAse I Millipore Sigma Cat# 11284932001 TryplE Fisher Scientific Cat# 10043382 FuGENE HD Promega Cat# E2311 RNAlater Sigma Aldrich Cat# #R0901 Phosphatase Inhibitor Cocktail II Merck Cat# P0044 Phosphatase Inhibitor Cocktail III Merck Cat# P5726 Critical commercial assays DNA extraction using Nucleobond Xtra-midi EF kit Macherey-Nagel Cat# 740420.50 Live/Dead assay using LIVE/DEADTM Fixable Far Red Dead Cell Stain Kit Invitrogen Cat# L34976 Live/Dead assay using LIVE/DEADTM Fixable Violet Dead Cell Stain Kit (Invitrogen, L34963) Invitrogen Cat# L34963 QIAquick Gel Extraction Kit Qiagen Qiagen, 28706 RNeasy mini kit Qiagen Qiagen, 74104 QIAquick PCR Purification Kit Qiagen Qiagen, 28104 QIAprep Spin Miniprep Kit Qiagen Qiagen, 27106 Quick Ligation Kit NEB NEB, M2200 CellTiter-Glo 3D luminescent assay Promega Cat# G9683 Deposited data Resource website for the sequencing data generated in this publication This paper ‘‘ENA: PRJEB85548’’ Sequence data, analyses, and resources related to the RNA sequencing and ChIP sequencing of the CDX models in this paper This paper ‘‘Zenodo Data: https://doi.org/ 10.5281/zenodo.14866517’’ Experimental models: Cell lines HCC33 human SCLC cell line Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat# ACC487; RRID: CVCL_2058 H1339 human SCLC cell line Creative Bioarray Cat# CSC-C0516; RRID: CVCL_A472 HT29 human colon adenocarcinoma cell line ATCC Cat# HTB-38; RRID: CVCL_A8EZ CDX cells This paper (Simpson et al.12; Pearsall et al.18) N/A LentiX 293T cells Clontech Cat# 632180 Experimental models: Organisms/strains Mice: NOD SCID Gamma (NSG) (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) Charles River (refreshed and bred in-house) Strain code 614 (Continued on next page) 20 Cell Reports 44, 115603, May 27, 2025



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